The materials in the record group mostly consist of reproductions of building plans of the Hadassah hospitals on Mount Scopus and Ein Kerem from the 1920s to the 2000s. Other properties documented in the record group include buildings managed by Youth Aliyah, Hadassah Youth Services, Young Judaea, Hadassah Israel Education Services, the National Office, and the Hadassah Medical Organization. These records document a core Hadassah function, the building of medical and social service facilities in Palestine/Israel.

This record group contains three-dimensional objects and printed materials that relate to the history of Hadassah. A bulk of this record group consists of promotional and commemorative objects and awards created by Hadassah for its Annual and Midwinter National Conventions, and for Young Judaea events. Examples of such items include t-shirts, hats, bags, buttons, stationery and keychains. Artifacts created by local Hadassah chapters and regions, as well as awards received by local and national Hadassah leaders from other organizations, are also included. Of a particular interest is the bronze death mask of Henrietta Szold.

This record group contains materials related to the local units of Hadassah—groups, chapters, regions, and co-ops—as well as Junior Hadassah, a youth organization that functioned as a group within the Hadassah Chapter structure. The record group documents over one hundred years of Hadassah’s growth, and illuminates a century of American Jewish communal life, particularly that of Jewish women, across the United States. The record group reflects the formation, administration and activities of the individual groups, chapters, co-ops and regions, and contains information on local events and programs organized around fundraising, Zionism, Jewish heritage, religion and holidays celebration, the study of Hebrew and Yiddish, women's issues, fashion, health, technology and many other topics.

This record group includes documents created and maintained by the Office of the President, the Office of the Executive Director and the Chair of the Division Coordinators/Directors Committee. Prominent is the Henrietta Szold series, containing correspondence by and to Szold as well as printed materials written by and about her. The files in this record group were created by a national president or executive director, or for their use, or maintained in their office during their years in office. Included are correspondence, minutes, memos, publications, reports and subject files on topics with which these individuals were involved.

The Hadassah Medical Organization Records in the Hadassah Archives document Hadassah's work in providing health care resources in Palestine/Israel since 1918. The activities documented revolve around the development of the Hadassah Hospital; health centers; dental centers; occupational and rehabilitative services; medical, nursing, dental, and pharmacy schools; as well as numerous educative and preventive projects, especially those aimed at infant care. The documents also reflect the history of the Yishuv (Jewish settlement in Palestine) and the establishment of the State of Israel. The record group contains articles, clippings, correspondence, financial records, fundraising material, minutes, personal accounts, press releases, publicity material, reports, and statistical reports.

The Hadassah Microfilm Collection consists of 16mm and 35mm reels containing images of various Hadassah publications, administrative documents, and personal archival material. Content includes on-site photographs, membership and organizational documents, regional chapter lists and histories, items relating to Hadassah’s Zionist political history, and a near-complete run of Hadassah Newsletter/Magazine. Other than the newsletter/magazine run, all images are unique within the collection.

The materials in this record group document the entirety of Hadassah’s history and work in Israel and the United States in photographs—prints, slides, glass lantern plates, and digital images.