Hadassah Functions and Operations Records represent the bulk of aid and services provided to Hadassah's membership, from the 1920s to 2011. Materials found in the record group include correspondence, clippings, press releases, manuals and kits for chapters and members to implement programming and chapter structure, fundraising campaigns, scripts, study guides, programming for local and national meetings, biographical files, and training documentation. Departments documented in the record group include Public Affairs, Education, Women's Health, American Affairs, Zionist and International Affairs, Speakers Bureau, Fundraising, Program, Organization, Outreach and Tourism Departments. Materials related to the general administration of Hadassah are also in the record group; these materials include research and development of projects, archives department correspondence and other materials, Hadassah House administration, and strategic planning. The record group also documents Hadassah's efforts to expand membership outside of the United States, by the development of Hadassah International.

The Name File record group is a collection of documents representing various individuals, corporations, and non-profit groups who were affiliated with Hadassah. These files were originally arranged alphabetically by the organization's central filing department as a ready reference source on leaders, doctors, speakers, donors, and religious figures associated with Hadassah's many projects. This collection includes correspondence, clippings, newsletters, and other ephemeral documents.

This record group contains personal papers and special collections documenting individuals, both Hadassah members and non-Hadassah members, who were important to Hadassah. Much of the material forming the collections in this record group came from the administrative files of the national office of Hadassah, though some of the material was donated to Hadassah. Key individuals represented within this record group include Hadassah national board members Anna Tulin Elyachar, Bertha S. Schoolman, and Denise Tourover Ezekiel, as well as Jesse Zel Lurie who served as the first professional editor of Hadassah Magazine (originally Hadassah Newsletter) from 1947 to 1980.

The Rahn Family Collection centers on the lives of Alfred and Lilli (née Bechmann) Rahn, but also contains many documents of their parents, siblings, and even more distant family members. It also documents the family members' attempts to receive restitution for their losses. The collection includes a large amount of correspondence, official, personal, and legal documents, photographs and photo albums, financial documentation, manuscripts and fragments of creative and academic writing, family trees and genealogical notes, newspaper clippings, poetry, educational certificates and diplomas, texts of lectures, teaching materials, a few recipes, and other papers.