This record group contains materials related to the local units of Hadassah—groups, chapters, regions, and co-ops—as well as Junior Hadassah, a youth organization that functioned as a group within the Hadassah Chapter structure. The record group documents over one hundred years of Hadassah’s growth, and illuminates a century of American Jewish communal life, particularly that of Jewish women, across the United States. The record group reflects the formation, administration and activities of the individual groups, chapters, co-ops and regions, and contains information on local events and programs organized around fundraising, Zionism, Jewish heritage, religion and holidays celebration, the study of Hebrew and Yiddish, women's issues, fashion, health, technology and many other topics.

This record group documents the creation, management, and use of the Hadassah Archives and provides valuable contextual information for researchers, current managers, and future managers of the Hadassah Archives. As an organization, Hadassah created active records of business that were housed in its Central Files department. In the 1950s, Hadassah began the process of creating an official archives, precipitated by a project to locate and compile sources for a biography of Hadassah founder, Henrietta Szold, on the occasion of the 100th anniversary of her birth. This record group documents three specific periods of custodianship—the 1980s, 1996-2011, and 2015-2016—and the various archivists who managed and shaped the archives during those years. RG 23 includes born digital, digitized, and paper material.

This record group documents the work of the Hadassah national board through board and executive committee meeting minutes, board member subject files, correspondence and reports, as well as minutes and materials generated by Hadassah ad-hoc and non-Executive committees, from 1912-2012. This record group also includes files from milestone anniversaries of Hadassah and legal documents pertaining to its projects.

This record group includes documents created and maintained by the Office of the President, the Office of the Executive Director and the Chair of the Division Coordinators/Directors Committee. Prominent is the Henrietta Szold series, containing correspondence by and to Szold as well as printed materials written by and about her. The files in this record group were created by a national president or executive director, or for their use, or maintained in their office during their years in office. Included are correspondence, minutes, memos, publications, reports and subject files on topics with which these individuals were involved.

The Hadassah Medical Organization Records in the Hadassah Archives document Hadassah's work in providing health care resources in Palestine/Israel since 1918. The activities documented revolve around the development of the Hadassah Hospital; health centers; dental centers; occupational and rehabilitative services; medical, nursing, dental, and pharmacy schools; as well as numerous educative and preventive projects, especially those aimed at infant care. The documents also reflect the history of the Yishuv (Jewish settlement in Palestine) and the establishment of the State of Israel. The record group contains articles, clippings, correspondence, financial records, fundraising material, minutes, personal accounts, press releases, publicity material, reports, and statistical reports.

The records document the Histadruth Ivrit's early history to the present, representing a significant portion of its work in spreading the Hebrew language in the United States in the second half of the twentieth-century. The records include substantial amount of material regarding the organization's history, administration, public events, publications, and reports. Some information of the early history of the Histadruth Ivrit could be found in the records kept by the writer Daniel Persky. Persky collected personal and professional records that include correspondence with friends, readers, and writers; a partial collection of the drafts of his own publications, and a collection of photographs and newspaper clippings. The functions and activities of the Histadruth Ivrit are documented through Board of Trustees and Board meetings agendas and minutes; various programs for events, conventions, conferences, and celebrations; documents related to fundraising; public relations, press releases and brochures; correspondence with different individuals, organizations, and foundations; Histadruth Ivrit's publications among them the newspaper Hadoar and Tov Lichtov; a large collection of photographs, and scrapbooks. The records of the Histadruth Ivrit represent the large majority of the organization's activities dating from the 1980s to the present. Records for the earlier years of activities are fragmented and incomplete. The records related to the life of Daniel Persky are also partial and copies of many of his publications are missing. This collection included brochures, correspondence, financial records, flyers, grant applications, invitations, lists, minutes, news clipping, orders, periodicals, photographs, press releases, reports, and scrapbooks.

The collection documents the National Jewish Welfare Board's (JWB) evolution from an organization founded in 1917 to provide support for soldiers in times of war to an agency involved in all aspects of Jewish life both in the United States and abroad. In 1990 JWB recreated itself as the Jewish Community Centers Association of North America.

This record group contains personal papers and special collections documenting individuals, both Hadassah members and non-Hadassah members, who were important to Hadassah. Much of the material forming the collections in this record group came from the administrative files of the national office of Hadassah, though some of the material was donated to Hadassah. Key individuals represented within this record group include Hadassah national board members Anna Tulin Elyachar, Bertha S. Schoolman, and Denise Tourover Ezekiel, as well as Jesse Zel Lurie who served as the first professional editor of Hadassah Magazine (originally Hadassah Newsletter) from 1947 to 1980.

The Rahn Family Collection centers on the lives of Alfred and Lilli (née Bechmann) Rahn, but also contains many documents of their parents, siblings, and even more distant family members. It also documents the family members' attempts to receive restitution for their losses. The collection includes a large amount of correspondence, official, personal, and legal documents, photographs and photo albums, financial documentation, manuscripts and fragments of creative and academic writing, family trees and genealogical notes, newspaper clippings, poetry, educational certificates and diplomas, texts of lectures, teaching materials, a few recipes, and other papers.

Young Judaea is the oldest Zionist youth organization in the United States, established as a national organization in 1909 by the Federation of American Zionists. It was supported by Hadassah, including direct financial sponsorship from 1967-2011. The major aims of Young Judaea throughout its history have been to advance the cause of Zionism, to further the mental, moral, and physical development of Jewish youth, and to promote Jewish culture and ideals in accordance with Jewish traditions. Young Judaea has remained non-partisan and non-denominational, embracing and recruiting Jewish youth from all backgrounds.

The Youth Aliyah Records in the Hadassah Archives document Hadassah's work with multiple international organizations to rescue Jewish children from continental Europe to Palestine from 1933-1945. The collection also documents Hadassah's involvement with Youth Aliyah since 1946 in providing residential, educational, vocational, rehabilitative and therapeutic care for displaced and at-risk youth from around the world.