This record group contains materials related to the local units of Hadassah—groups, chapters, regions, and co-ops—as well as Junior Hadassah, a youth organization that functioned as a group within the Hadassah Chapter structure. The record group documents over one hundred years of Hadassah’s growth, and illuminates a century of American Jewish communal life, particularly that of Jewish women, across the United States. The record group reflects the formation, administration and activities of the individual groups, chapters, co-ops and regions, and contains information on local events and programs organized around fundraising, Zionism, Jewish heritage, religion and holidays celebration, the study of Hebrew and Yiddish, women's issues, fashion, health, technology and many other topics.

This record group contains meeting minutes, correspondence and reports of the Hadassah Council in Israel (originally the Hadassah Emergency Services and the Hadassah Council in Palestine), the Hadassah Youth Services (originally the Palestine Council of Hadassah) and the Hadassah Youth Services (HYS) successor organizations, Hadassah Vocational Education Services (HVES) and Hadassah Israel Education Services (HIES). The records represent the activities of Hadassah's representatives in Palestine/Israel, from 1927 to the 1990s. The Hadassah Youth Services focused on providing services to underserved youth in Palestine/Israel, most notably with their school luncheon and Guggenheimer playground programs. After HYS changed its working name to HVES in 1952, it began to focus on vocational education projects in Israel. Legally, however, the name of the organization in Israel remained Hadassah Youth Services. The Hadassah Council in Israel acted as an advisor and liaison between Hadassah's American offices and Hadassah's Israel projects, including the Hadassah Medical Organization, Youth Aliyah, and Hadassah Youth Services.

The Hadassah Medical Organization Records in the Hadassah Archives document Hadassah's work in providing health care resources in Palestine/Israel since 1918. The activities documented revolve around the development of the Hadassah Hospital; health centers; dental centers; occupational and rehabilitative services; medical, nursing, dental, and pharmacy schools; as well as numerous educative and preventive projects, especially those aimed at infant care. The documents also reflect the history of the Yishuv (Jewish settlement in Palestine) and the establishment of the State of Israel. The record group contains articles, clippings, correspondence, financial records, fundraising material, minutes, personal accounts, press releases, publicity material, reports, and statistical reports.

The Youth Aliyah Records in the Hadassah Archives document Hadassah's work with multiple international organizations to rescue Jewish children from continental Europe to Palestine from 1933-1945. The collection also documents Hadassah's involvement with Youth Aliyah since 1946 in providing residential, educational, vocational, rehabilitative and therapeutic care for displaced and at-risk youth from around the world.