The Hadassah subject file record group is a collection of files of organizations, events, and genre subjects originally arranged alphabetically by Hadassah’s central filing department. These files served and serve as a ready reference source that represents both the direct and indirect involvement of Hadassah in both national and international affairs. This collection includes correspondence, clippings, newsletters, photographs, and other ephemeral documents.

The material in this record group was culled from Hadassah's Central Files in Israel in the early 1980s to document Hadassah's role in Zionist history. Originally formed from a Zionist women's study group, the first Hadassah chapter in New York had a strong relationship with the Zionist Organization of America (ZOA; then known as the Federation of American Zionists). The material in this record group documents Hadassah's relationship to the ZOA and to other Zionist organizations in the United States, Europe, and Palestine/Israel, particularly in the years leading up to Israeli statehood in 1948. Other subjects addressed in this record group include the founding of Hadassah; World War II, particularly relating to Jewish emigration and refugees; the founding of the United Nations and the debate over recognition of a Jewish state; the partition of Palestine; and Arab-Jewish relations. Included are articles, clippings, convention resolutions, correspondence, diary extracts, memorandums, minutes, press releases, printed ephemera, publications, reports, and speeches.