This record group includes documents created and maintained by the Office of the President, the Office of the Executive Director and the Chair of the Division Coordinators/Directors Committee. Prominent is the Henrietta Szold series, containing correspondence by and to Szold as well as printed materials written by and about her. The files in this record group were created by a national president or executive director, or for their use, or maintained in their office during their years in office. Included are correspondence, minutes, memos, publications, reports and subject files on topics with which these individuals were involved.

The Hadassah Medical Organization Records in the Hadassah Archives document Hadassah's work in providing health care resources in Palestine/Israel since 1918. The activities documented revolve around the development of the Hadassah Hospital; health centers; dental centers; occupational and rehabilitative services; medical, nursing, dental, and pharmacy schools; as well as numerous educative and preventive projects, especially those aimed at infant care. The documents also reflect the history of the Yishuv (Jewish settlement in Palestine) and the establishment of the State of Israel. The record group contains articles, clippings, correspondence, financial records, fundraising material, minutes, personal accounts, press releases, publicity material, reports, and statistical reports.

The Hadassah Microfilm Collection consists of 16mm and 35mm reels containing images of various Hadassah publications, administrative documents, and personal archival material. Content includes on-site photographs, membership and organizational documents, regional chapter lists and histories, items relating to Hadassah’s Zionist political history, and a near-complete run of Hadassah Newsletter/Magazine. Other than the newsletter/magazine run, all images are unique within the collection.

The materials in this record group document the entirety of Hadassah’s history and work in Israel and the United States in photographs—prints, slides, glass lantern plates, and digital images.

The Youth Aliyah Records in the Hadassah Archives document Hadassah's work with multiple international organizations to rescue Jewish children from continental Europe to Palestine from 1933-1945. The collection also documents Hadassah's involvement with Youth Aliyah since 1946 in providing residential, educational, vocational, rehabilitative and therapeutic care for displaced and at-risk youth from around the world.